Radiation-induced mutagenicity and lethality in Salmonella typhimurium
The mutagenic and lethal effects of ionizing radiation on histidine-deficient auxotrophs of Salmonella typhimurium were studied to improve the understanding of radiation damage to DNA. The auxotrophs were divided into two groups - one which is sensitive to base-pair substitutions and another sensitive to frameshifts. These groups were composed of parent-daughter pairs in which the chemical mutagenicity enhancing plasmid, pKM101, is absent in the parent strain and present in the daughter. Co-60 ..gamma..-radiation and 250 kV x-rays were used to irradiate the bacteria. Irradiation of the frameshift - sensitive strains which carry the pKm101 plasmid doubled the absolute number of induced revertants whereas irradiation of the base-pair substitution sensitive strain which also carries the pKm101 plasmid produced nearly no change in the number of induced revertants. A nearly negligible effect on the mutation rate was observed for all parent strains. (ACR)
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA). Div. of Radiation Biology
- DOE Contract Number:
- AC02-78EV04746
- OSTI ID:
- 5986380
- Report Number(s):
- CONF-830710-3; ON: DE83014653
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
INFORMATION
IONIZING RADIATIONS
LETHAL DOSES
MICROORGANISMS
MUTAGENESIS
MUTATION FREQUENCY
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
SALMONELLA
SALMONELLA TYPHIMURIUM
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
DATA
DNA
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
INFORMATION
IONIZING RADIATIONS
LETHAL DOSES
MICROORGANISMS
MUTAGENESIS
MUTATION FREQUENCY
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
SALMONELLA
SALMONELLA TYPHIMURIUM
X RADIATION