Culture conditions affecting the survival response of Chinese hamster ovary cells treated by hyperthermia

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · NIDA Res. Monogr.; (United States)

OSTI ID:5985170

Highfield, D P; Holahan, E V; Dewey, W C

Using lethally irradiated feeder cells to control cell population densities, researchers investigated the survival of Chinese hamster ovary cells heated between 42.2 and 45.5 degrees C. Test cells were plated into T25 flasks with or without feeder cells, incubated 2 hours at 37 degrees C, and then given various heat treatments. Under all heating conditions, survival increased in those flasks containing feeder cells. Increased survival (by as much as a factor of 100 for cells heated at 42.4 degrees C for 6-10 hr) was most apparent when cells were heated to thermotolerance. By adjustment of test and feeder cell numbers, survival increased as density increased; however, maximum survival followed a transition period that occurred between the plating of 1 X 10(4) and 6 X 10(4) cells. Experimental artifacts due to improper control of cell density was demonstrated.

• Research Organization: Department of Radiology and Radiation Biology, Colorado State University, Ft. Collins
• OSTI ID: 5985170
• Journal Information: NIDA Res. Monogr.; (United States), Journal Name: NIDA Res. Monogr.; (United States) Vol. 61; ISSN MIDAD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560201 -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BODY TEMPERATURE
CELL CULTURES
EXTERNAL IRRADIATION
FEMALE GENITALS
GONADS
HAMSTERS
HYPERTHERMIA
IRRADIATION
MAMMALS
NORTH AMERICA
ORGANS
OVARIES
POPULATION DYNAMICS
RADIATION EFFECTS
RESPONSE MODIFYING FACTORS
RODENTS
SURVIVAL CURVES
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TOLERANCE
USA
VERTEBRATES