Interaction of hyperthermia and radiation on the induction of division delay in Chinese hamster ovary cells

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · NIDA Res. Monogr.; (United States)

OSTI ID:5985148

Kimler, B F

The mitotic selection procedure for cell cycle analysis was used in the investigation of the interaction of hyperthermia and ionizing radiation on the induction and duration of division delay in Chinese hamster ovary cells. Hyperthermia (immersion in a 45 degrees C water bath) produced a blockade of cell cycle progression with a transition point in late G2-early M, approximately at the X-ray transition point (35 min prior to selection). The duration of division delay for heated cells depended on the time of immersion: 24 minutes/minute at 45 degrees C. Radiation-induced division delay occurred at a rate of 45 minutes/gray of X-irradiation. When hyperthermic exposure and X-irradiation were combined with less than 1 minute between treatments, a division delay resulted that was approximately the sum of the delays produced by the individual treatments. As the interval between treatments was increased, the overall division delay also increased beyond that which could be accounted for solely by the postponement of the second treatment. These results indicate that hyperthermia and radiation induce division delay by different mechanisms.

• Research Organization: Department of Radiation Therapy, University of Kansas Medical Center, Kansas City
• OSTI ID: 5985148
• Journal Information: NIDA Res. Monogr.; (United States), Journal Name: NIDA Res. Monogr.; (United States) Vol. 61; ISSN MIDAD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BODY TEMPERATURE
CELL CULTURES
CELL CYCLE
CELL DIVISION
COMPARATIVE EVALUATIONS
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
FRACTIONATED IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GONADS
HAMSTERS
HYPERTHERMIA
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MEDICINE
MITOSIS
NORTH AMERICA
NUCLEAR MEDICINE
ORGANS
OVARIES
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RADIOTHERAPY
RESPONSE MODIFYING FACTORS
RODENTS
TEMPERATURE EFFECTS
THERAPY
USA
VERTEBRATES
X RADIATION