Correction of fluorescence detected circular dichroic photoselection artifacts using light collecting ellipsoidal mirrors
Ellipsoidal mirrors are used to collect the emitted light from fluorophores in fluorescence detected circular dichroic (FDCD) measurements and to deliver this light to a photomultiplier tube (PMT) at 90/sup 0/ to the excitation beam. These mirrors collect light over a symmetrical 71% of the emission sphere centered on the sample cell, and thereby eliminate most of the nonuniformity in detected emission resulting from the anisotropy of photoselection. Furthermore, even with reflectance and path length losses, the amount of fluorescent emission reaching the PMT is increased by a factor of approximately 18. Elimination of photoselection artifacts in UV FDCD spectra is demonstrated by comparing spectra of L-tryptophan in glycerol with its spectra in water. Validity of the values measured is verified through calibration with a d10 camphorsulfonic acid (d10 CSA) standard.
- Research Organization:
- Department of Biophysics, University of California, Berkeley, California 94720
- OSTI ID:
- 5984767
- Journal Information:
- Rev. Sci. Instrum.; (United States), Journal Name: Rev. Sci. Instrum.; (United States) Vol. 58:11; ISSN RSINA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Portable biochip scanner device
Further developments of a microscope-based flow cytometer: light scatter detection and excitation intensity compensation
Related Subjects
400102* -- Chemical & Spectral Procedures
440300 -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
BIOCHEMISTRY
CALIBRATION
CHEMISTRY
DESIGN
DICHROISM
EMISSION SPECTROSCOPY
EQUIPMENT
FLUORESCENCE
FLUORESCENCE SPECTROSCOPY
LASER SPECTROSCOPY
LAWRENCE BERKELEY LABORATORY
LUMINESCENCE
MAGNETIC CIRCULAR DICHROISM
MIRRORS
MOLECULES
NATIONAL ORGANIZATIONS
OPTICAL EQUIPMENT
PHOTOCHEMISTRY
POLARIMETERS
SAMPLE HOLDERS
SPECTROSCOPY
US AEC
US DOE
US ERDA
US ORGANIZATIONS