Effects of culture age on symbiotic infectivity of Rhizobium japonicum
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5983160
The infectivity of the soybean symbiont Rhizobium japonicum changed two- to fivefold with culture age for strains 110 ARS, 138 Str Spc, and 123 Spc, whereas culture age had relatively little effect on the infectivity of strains 83 Str and 61A76 Str. Infectivity was measured by determining the number of nodules which developed on soybean primary roots in the zone which contained developing and preemergent root hairs at the time of inoculation. Root cells in this region of the host root are susceptible to Rhizobium infection, but this susceptibility is lost during acropetal development and maturation of the root cells within a period of 4 to 6 h. Profiles of nodulation frequency at different locations on the root were not affected by the age of the R. japonicum cultures, indicating that culture age affected the efficiency of Rhizobium infection rather than how soon infections were initiated after inoculation. Inoculum dose-response experiments also indicated that culture age affected the efficiency of infection. Two strains, 61A76 Str and 83 Str, were relatively inefficient at all culture ages, particularly at low inoculum doses. Changes in infectivity with culture age were reasonably well correlated with changes in the proportion of cells in a culture capable of binding soybean lectin. Suspensions of R. japonicum in water were found to retain their viability and infectivity. 15 references, 6 figures, 2 tables.
- Research Organization:
- Charles F. Kettering Research Lab., Yellow Springs, OH
- DOE Contract Number:
- AC02-81ER10895
- OSTI ID:
- 5983160
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 153:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Basis for the competitiveness of rhizobium japonicum in nodulation of soybean. Final progress report
Role of pili (fimbriae) in attachment of Bradyrhizobium japonicum to soybean roots
(Basis for the competitiveness of Rhizobium japonicum in nodulation of soybean). Progress report, 1984
Technical Report
·
Mon Jul 30 00:00:00 EDT 1984
·
OSTI ID:6579723
Role of pili (fimbriae) in attachment of Bradyrhizobium japonicum to soybean roots
Journal Article
·
Tue Jul 01 00:00:00 EDT 1986
· Appl. Environ. Microbiol.; (United States)
·
OSTI ID:5014283
(Basis for the competitiveness of Rhizobium japonicum in nodulation of soybean). Progress report, 1984
Technical Report
·
Sat Dec 31 23:00:00 EST 1983
·
OSTI ID:5006040