Pyruvate orthophosphate dikinase gene expression in developing wheat seeds

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.75.2.393· OSTI ID:5975787

Aoyagi, K; Bassham, J A; Greene, F C

The amount of pyruvate orthophosphate dikinase (PPDK) (EC 2.7.9.1) protein in wheat (Triticum aestivum L. var Cheyenne) grains was determined at different stages of development by the protein blot method. The variation in PPDK protein with time in developing wheat grains was similar to that of the enzyme's activity reported by Meyer et al. The variation in levels of PPDK mRNA with seed development was determined by analysis of polypeptides immunoprecipitated by anti-PPDK serum from in vitro translation products of extracted seed RNA. This mRNA variation was similar to that of the in vivo enzyme levels and the correlation is consistent with the regulation of PPDK gene expression by the level of its mRNA. The highest level of PPDK in developing wheat seeds occurs later than the highest levels of both ribulose bisphosphate carboxylase and of chlorophyll, which are located in the green pericarp tissue. PPDK was located in both endosperm and pericarp tissue of the seeds. The tissue location and development profile of seed PPDK are consistent with a metabolic role of providing phosphoenolpyruvate as a substrate for recapturing respiratory CO/sub 2/ in the seed, and possibly for amino acid interconversion during development.

• Research Organization: Lawrence Berkeley Lab., CA
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5975787
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 75:2; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BODY
CARBOXYLASE
CARBOXYLIC ACIDS
CEREALS
CHLOROPHYLL
ENZYME ACTIVITY
ENZYMES
GENES
GRASS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LIGASES
MESSENGER-RNA
METABOLISM
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYTOCHROMES
PIGMENTS
PLANT TISSUES
PLANTS
PORPHYRINS
PROTEINS
RNA
SEEDS
TISSUES
TRANSFERASES
WHEAT