Cyclization of farnesyl pyrophosphate to the sesquiterpene olefins humulene and caryophyllene by an enzyme system from sage (Salvia officinalis)
Journal Article
·
· Arch. Biochem. Biophys.; (United States)
A soluble enzyme preparation obtained from sage (Salvia officinalis) leaves was shown to catalyze the divalent metal-ion dependent cyclization of trans, trans-farnesyl pyrophosphate to the macrocyclic sesquiterpene olefins humulene and caryophyllene. The identities of the biosynthetic products were confirmed by radiochromatographic analysis and by preparation of crystalline derivatives, and the specificity of labeling in the cyclization reaction was established by chemical degradation of the olefins derived enzymatically from (1-3H2)farnesyl pyrophosphate. These results constitute the first report on the cyclization of farnesyl pyrophosphate to humulene and caryophyllene, two of the most common sesquiterpenes in nature, and the first description of a soluble sesquiterpene cyclase to be isolated from leaves of a higher plant.
- Research Organization:
- Washington State Univ., Pullman
- OSTI ID:
- 5975305
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 233:2; ISSN ABBIA
- Country of Publication:
- United States
- Language:
- English
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Wed Jul 01 00:00:00 EDT 1987
· Arch. Biochem. Biophys.; (United States)
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OSTI ID:6132900
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Mon Jul 25 00:00:00 EDT 1988
· J. Biol. Chem.; (United States)
·
OSTI ID:6829335
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKENES
BIOSYNTHESIS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DEHYDROCYCLIZATION
ENZYMES
HERBS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
PYROPHOSPHATES
RADIOCHEMISTRY
RADIOCHROMATOGRAPHY
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ALKENES
BIOSYNTHESIS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
DEHYDROCYCLIZATION
ENZYMES
HERBS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
PYROPHOSPHATES
RADIOCHEMISTRY
RADIOCHROMATOGRAPHY
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS