Non-destructive monitoring of microbial biofilms at solid-liquid interfaces using on-line devices

Conference · Mon Jan 01 00:00:00 EST 1990

OSTI ID:5973556

Nivens, D E ^[1]; Chambers, J Q ^[2]; White, D C ^[3]

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1. Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry Tennessee Univ., Knoxville, TN (USA). Inst. for Applied Microbiology
2. Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry
3. Tennessee Univ., Knoxville, TN (USA). Inst. for Applied Microbiology Tennessee Univ., Knoxville, TN (USA). Dept. of Microbiology Oak Ridge National Lab., TN (USA)

Corrosion, biofouling, and related problems have been an impetus for investigating interactions between microorganisms and solid surfaces. In recent years, a number of studies have been performed to assess the damages caused by microbial influenced corrosion (MIC). In a number of these studies, electrochemical techniques have monitored the performance of metal surfaces exposed to bacteria. However, most of these methods can only indirectly detect the presence of biofilms. In this paper, two non-destructive on-line monitoring devices, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT/IR) and the quartz crystal microbalance (QCM) were used to directly monitor biofilm formation. These devices have been developed to study the initial fouling process and subsequent biofilm development and not merely the effects of the living film on the host material. The ATR-FT/IR technique provides information about biomass, exopolymer production, and the nutritional status of microbial biofilms. The QCM provides a direct measure of biomass. ATR-FT/IR and QCM detect 10{sup 6} and 10{sup 4} Caulobacter crescentus cells/cm{sup 2}, respectively. Both techniques can be coupled with electrochemical methods for deeper insight into mechanisms of MIC. 20 refs., 2 figs.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOD; USDOE; National Science Foundation (NSF); Department of Defense, Washington, DC (USA); USDOE, Washington, DC (USA); National Science Foundation, Washington, DC (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5973556
• Report Number(s): CONF-9010138-3; ON: DE91010777; CNN: N000014-88-K-01777; CHE-8718057
• Resource Relation: Conference: International congress on microbially influenced corrosion, Knoxville, TN (USA), 7-12 Oct 1990
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
36 MATERIALS SCIENCE
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
NONDESTRUCTIVE ANALYSIS
FILMS
METALS
BIOLOGICAL FOULING
CORROSION
BIOMASS
COPPER
ELECTROCHEMISTRY
FOURIER TRANSFORM SPECTROMETERS
INFRARED SPECTRA
INTERFACES
MONITORING
NUTRITION
POLYMERS
SURFACE AREA
SURFACE COATING
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHEMISTRY
DEPOSITION
ELEMENTS
ENERGY SOURCES
FOULING
MEASURING INSTRUMENTS
MICROORGANISMS
RENEWABLE ENERGY SOURCES
SPECTRA
SPECTROMETERS
SURFACE PROPERTIES
TRANSITION ELEMENTS
400102* - Chemical & Spectral Procedures
360105 - Metals & Alloys- Corrosion & Erosion
550700 - Microbiology