Enzymic activities of cadmium- and zinc-tolerant strains of Klebsiella (Aerobacter) aerogenes growing in glucose-limited chemostats
The activities of phosphatases and some enzymes of glucose metabolism were determined in K. aerogenes NCIB 418 and in two strains derived from it, resistant to 50 ..mu..g Cd/sup 2 +/ ml/sup -1/ and 16..mu..g Zn/sup 2 +/ ml/sup -1/ respectively, during growth at D = 0.38 h/sup -1/ in medium containing ..beta..-glycerophosphate as sole phosphorus source and supplemented with Cd/sup 2 +/ and Zn/sup 2 +/, as appropriate for the resistant strains. The pH-activity profiles of the phosphatases differed from strain to strain but all showed maximum activity at an acid pH and this activity was very much lower in the Zn/sup 2 +/-resistant strain than in the control and even lower in the Cd/sup 2 +/-resistant strain. Resistance to either metal was associated with decreased glucose-6-phosphate dehydrogenase activity and increased phosphoglucose isomerase activity, suggesting an increased flow of carbon through the Embden-Meyerhof pathway relative to the pentose phosphate pathway, but the efficiency of the conversion of glucose into biomass was largely unaffected. Glucose phosphoenolpyruvate phosphotransferase activity was also lower in the resistant strains. 25 references, 1 figure, 1 table.
- Research Organization:
- Univ. of Oxford, England
- OSTI ID:
- 5972729
- Journal Information:
- Microbios; (United States), Vol. 15:60
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
METABOLISM
ENZYME ACTIVITY
CORRELATIONS
TOLERANCE
BIOLOGICAL EFFECTS
CADMIUM
COMPARATIVE EVALUATIONS
EXPERIMENTAL DATA
PH VALUE
PHOSPHATASES
ZINC
DATA
ELEMENTS
ENZYMES
ESTERASES
HYDROLASES
INFORMATION
METALS
MICROORGANISMS
NUMERICAL DATA
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)