Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase

Patent · Tue Mar 03 00:00:00 EST 1998

OSTI ID:597119

Houtz, R L

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) {epsilon}N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the {epsilon}-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed. 5 figs.

• Research Organization: Univ. of Kentucky, Lexington, KY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG05-92ER20075
• Assignee: Univ. of Kentucky, Lexington, KY (United States)
• Patent Number(s): US 5,723,752/A/
• Application Number: PAN: 8-391,000
• OSTI ID: 597119
• Resource Relation: Other Information: PBD: 3 Mar 1998
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA-CLONING
TRANSFERASES
DNA SEQUENCING
PISUM
GENE REGULATION