Distribution, metabolism and excretion of N-nitrosobis(2-hydroxypropyl)amine in Wistar rats

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/5.11.1443· OSTI ID:5970236

Mori, Y; Takahashi, H; Yamazaki, H; Toyoshi, K; Makino, T; Yokose, Y; Konishi, Y

The metabolic fate of the carcinogen N-nitrosobis(2-hydroxypropyl)amine (BHP) in male Wistar rats was studied. The blood level of (1-/sup 14/C)BHP after a single intraperitoneal injection, administered at a carcinogenic dose of 3 g/kg body weight, reached a maximum within 1 h. Whereas a relatively high concentration of /sup 14/C was found in the blood and target organs, such as the lung, liver, thyroid gland and kidney 1 h after the treatment, most of the radioactive labelling had disappeared from the tissues by 24 h after injection. Most of the administered /sup 14/C was eliminated via the urine; 90.8% was excreted in the urine within the 24 h period, 5.5% in the feces and 3.2% by way of expired air. Studies in rats with exteriorized bile flow demonstrated that about 11% of the intraperitoneally administered /sup 14/C was excreted via the bile in 24 h. Analysis by h.p.l.c. detected BHP (78.1% of the dose), HPOP (1.5%), glucuronides of BHP (4.3%) and HPOP (0.16%), MHP (0.03%) and unknown metabolites (6.0%) in the urine 24 h after the treatment. Besides these metabolites, BOP and two unidentified metabolites were also detected in the blood, lung, liver or kidney of rats 3 h after the treatment. These results suggest the involvement of BHP metabolites, HPOP, MHP and BOP, in carcinogenesis and in particular lung carcinogenesis induced by BHP in rats.

• Research Organization: Gifu Pharmaceutical Univ., Japan
• OSTI ID: 5970236
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 5:11; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BILE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARCINOGENESIS
CARCINOGENS
CLEARANCE
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
EXCRETION
FECES
GLANDS
KIDNEYS
LABELLED COMPOUNDS
LIVER
LUNGS
MAMMALS
MATERIALS
METABOLISM
METABOLITES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
RATS
RESPIRATORY SYSTEM
RODENTS
THYROID
TISSUE DISTRIBUTION
URINE
VERTEBRATES
WASTES