Dog pulmonary macrophage metabolism of free and particle-associated (/sup 14/C)benzo(a)pyrene

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398409530572· OSTI ID:5968548

Bond, J A; Butler, M M; Medinsky, M A; Muggenburg, B A; McClellan, R O

Pulmonary macrophages (PM) are involved in the clearance of inhaled particulate matter from the lung. PM also are capable of metabolizing xenobiotics such as benzo(a)pyrene (BaP). The objective of this investigation was to measure the ability of PM isolated from dogs to metabolize BaP coated onto diesel exhaust particles and to compare this metabolism with that of BaP in solution. Pm were isolated from male beagle dogs and incubated with 1 ..mu..M (/sup 14/C)BaP (solution or diesel particle coated) for select times up to 48 h. After incubation of PM with (/sup 14/C)BaP, both the cells and the media were individually analyzed for (/sup 14/C)BaP metabolites by high-performance liquid chromatography. Total quantities of (/sup 14/C)BaP metabolites in both the media (125 pmol/10/sup 6/ cells) and cells (45 pmol/10/sup 6/ cells) increased with incubation time for up to 48 h. BaP-9,10-diol and BaP-7,8-diol were the major metabolites in organic extracts from the culture media, whereas BaP-7,8-diol and BaP-4,5-diol were the major metabolites in extracts of cells. Small quantities of BaP phenols and BaP quinones were detected in both the cells and media. Total quantities of BaP metabolites (20-30 pmol/10/sup 6/) were not significantly different when PM were incubated for 24 h with either (/sup 14/C)BaP in solution or (/sup 14/C)BaP coated on diesel particles. The data suggest that particles retained in lungs are capable of being acted upon by PM metabolizing enzymes and that the ensuing metabolism may play an important role in the metabolic fate of organic material inhaled on particulate matter. 23 references, 3 figures.

• Research Organization: Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM
• DOE Contract Number: AC04-76EV01013
• OSTI ID: 5968548
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 14:2-3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BEAGLES
BENZOPYRENE
BIOLOGICAL FUNCTIONS
BODY
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DATA
DIESEL ENGINES
DOGS
ENGINES
ENZYME ACTIVITY
EXHAUST GASES
EXPERIMENTAL DATA
FLUIDS
FUNCTIONS
GASEOUS WASTES
GASES
HEAT ENGINES
HYDROCARBONS
INFORMATION
INHALATION
INTAKE
INTERNAL COMBUSTION ENGINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MACROPHAGES
MAMMALS
METABOLISM
METABOLITES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PARTICLES
PARTICULATES
PHAGOCYTES
RESPIRATORY SYSTEM
RETENTION
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES
WASTES