Construction and expression of a recombinant DNA gene encoding a polyomavirus middle-size tumor antigen with the carboxyl terminus of the vesicular stomatitis virus glycoprotein G
Journal Article
·
· Mol. Cell. Biol.; (United States)
The authors constructed a molecular clone encoding the N-terminal 379 amino acids of the polyomavirus middle-size tumor antigen, followed by the C-terminal 60 amino acids of the vesicular stomatitis virus glycoprotein G. This hybrid gene contained the coding region for the C-terminal hydrophobic membrane-spanning domain of the G protein in place of the C-terminal hydrophobic domain of the middle-size tumor antigen. The hybrid gene was expressed in COS-1 cells under the control of the simian virus 40 late promoter. The hybrid protein was located in cell membranes and was associated with a tyrosine-specific protein kinase activity, as was the middle-size tumor antigen. Plasmids encoding the hybrid protein failed to transform mouse NIH 3T3 or rat F2408 cells.
- Research Organization:
- Molecular Biology and Virology Lab., The Salk Institute, San Diego, CA 92138
- OSTI ID:
- 5965337
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:2; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGENS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
CONTROL
DIGESTIVE SYSTEM
DISEASES
DNA
DNA-CLONING
ENZYME ACTIVITY
ENZYMES
GENES
GENETIC CONTROL
GLUCOPROTEINS
HYBRIDIZATION
HYDROXY ACIDS
INFLAMMATION
MEMBRANES
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORAL CAVITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
PEST CONTROL
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLASMIDS
POLYOMA VIRUS
PROTEINS
RECOMBINANT DNA
SACCHARIDES
SIMIAN VIRUS
SIZE
STRUCTURAL MODELS
SURFACE PROPERTIES
SYMPTOMS
TRANSFERASES
TYROSINE
VIRUSES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGENS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CLONING
CONTROL
DIGESTIVE SYSTEM
DISEASES
DNA
DNA-CLONING
ENZYME ACTIVITY
ENZYMES
GENES
GENETIC CONTROL
GLUCOPROTEINS
HYBRIDIZATION
HYDROXY ACIDS
INFLAMMATION
MEMBRANES
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORAL CAVITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
PEST CONTROL
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PLASMIDS
POLYOMA VIRUS
PROTEINS
RECOMBINANT DNA
SACCHARIDES
SIMIAN VIRUS
SIZE
STRUCTURAL MODELS
SURFACE PROPERTIES
SYMPTOMS
TRANSFERASES
TYROSINE
VIRUSES