Estradiol in saliva for monitoring follicular stimulation in an in vitro fertilization program

Journal Article · Sun Sep 01 00:00:00 EDT 1985 · Fert. Steril.; (United States)

OSTI ID:5965329

Belkien, L D; Bordt, J; Moeller, P; Hano, R; Nieschlag, E

A rapid and sensitive radioimmunoassay (RIA) was developed to compare serum and saliva estradiol (E/sub 2/) levels in patients undergoing ovulation induction in an in vitro fertilization and embryo transfer (IVF-ET) program. Serum and saliva E/sub 2/ were compared in 23 patients. The sensitivity of the saliva RIA standard curve was 11 fmol/tube (equal to 3.2 pg/tube). There was a highly significant correlation between serum and saliva E/sub 2/ throughout the stimulated cycles. The ratio of serum to saliva E/sub 2/ was constant throughout the stimulated cycles. The E/sub 2/ concentration per follicle was 1548 pmol/l in serum and 23 pmol/l in saliva. Mean E/sub 2/ levels in saliva (+/- SD) were 74 +/- 21 pmol/l at midcycle and 46 +/- 12 pmol/l at midluteal phase. The findings indicate that measurement of saliva E/sub 2/ provides a reliable, noninvasive method and may replace serum measurements for monitoring stimulated cycles in an IVF-ET program.

• Research Organization: Univ. Women's Hospital, Muenster, West Germany
• OSTI ID: 5965329
• Journal Information: Fert. Steril.; (United States), Vol. 44:3
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
ESTRADIOL
RADIOIMMUNOASSAY
OVULATION
STIMULATION
REPRODUCTIVE DISORDERS
DIAGNOSIS
BODY FLUIDS
FERTILITY
FERTILIZATION
IN VITRO
PATIENTS
BIOLOGICAL MATERIALS
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MATERIALS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
551001 - Physiological Systems- Tracer Techniques