Estradiol in saliva for monitoring follicular stimulation in an in vitro fertilization program

Journal Article · Sun Sep 01 04:00:00 EDT 1985 · Fert. Steril.; (United States)

OSTI ID:5965329

Belkien, L D; Bordt, J; Moeller, P; Hano, R; Nieschlag, E

A rapid and sensitive radioimmunoassay (RIA) was developed to compare serum and saliva estradiol (E/sub 2/) levels in patients undergoing ovulation induction in an in vitro fertilization and embryo transfer (IVF-ET) program. Serum and saliva E/sub 2/ were compared in 23 patients. The sensitivity of the saliva RIA standard curve was 11 fmol/tube (equal to 3.2 pg/tube). There was a highly significant correlation between serum and saliva E/sub 2/ throughout the stimulated cycles. The ratio of serum to saliva E/sub 2/ was constant throughout the stimulated cycles. The E/sub 2/ concentration per follicle was 1548 pmol/l in serum and 23 pmol/l in saliva. Mean E/sub 2/ levels in saliva (+/- SD) were 74 +/- 21 pmol/l at midcycle and 46 +/- 12 pmol/l at midluteal phase. The findings indicate that measurement of saliva E/sub 2/ provides a reliable, noninvasive method and may replace serum measurements for monitoring stimulated cycles in an IVF-ET program.

• Research Organization: Univ. Women's Hospital, Muenster, West Germany
• OSTI ID: 5965329
• Journal Information: Fert. Steril.; (United States), Journal Name: Fert. Steril.; (United States) Vol. 44:3; ISSN FESTA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOLOGICAL MATERIALS
BODY FLUIDS
DIAGNOSIS
ESTRADIOL
ESTRANES
ESTROGENS
FERTILITY
FERTILIZATION
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
ISOTOPE APPLICATIONS
MATERIALS
ORGANIC COMPOUNDS
OVULATION
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REPRODUCTIVE DISORDERS
STEROID HORMONES
STEROIDS
STIMULATION
TRACER TECHNIQUES