SEM and x-ray microanalysis of cellular differentiation in Sea Urchin Embryos: a frozen hydrated study

Technical Report · Sun Dec 01 04:00:00 EST 1985

DOI:https://doi.org/10.2172/5964745· OSTI ID:5964745

Klein, S B

Quantitative studies of major chemical element distribution among individual differentiating cells were attempted using scanning electron microscopy. Frozen hydrated embryos of the sea urchin Strongelocentrotus purpuratus were examined at three stages: blastula, mesenchyme blastula, and early gastrula. The blastocoel matrix contained large beads of approximately 1 ..mu..m diameter. The cells of the archenteron lacked well defined cell boundaries. Characteristic levels of beam damage and charging provided structural information. The primary mesenchyme cells within the blastocoel were particularly susceptible to both effects. Damaging effects were noted in material stored in liquid nitrogen longer than three months. Ice crystal growth, shrinkage, elemental shift, density changes and charge accumulation may take place in these stored specimens. 151 refs., 50 figs., 3 tabs.

• Research Organization: Lawrence Berkeley Lab., CA (USA)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 5964745
• Report Number(s): LBL-21128; ON: DE86009080
• Country of Publication: United States
• Language: English

Related Subjects

440103 -- Radiation Instrumentation-- Nuclear Spectroscopic Instrumentation
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
550300 -- Cytology
550800* -- Morphology
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGY
CELL DIFFERENTIATION
CHEMICAL ANALYSIS
CRYOBIOLOGY
CYTOLOGY
ECHINODERMS
ELECTRON MICROPROBE ANALYSIS
ELECTRON MICROSCOPY
ELEMENTS
EMBRYONIC CELLS
INVERTEBRATES
MAGNESIUM
METALS
MICROANALYSIS
MICROSCOPY
MORPHOLOGICAL CHANGES
NONMETALS
ONTOGENESIS
PHOSPHORUS
POTASSIUM
SCANNING ELECTRON MICROSCOPY
SEA URCHINS
SULFUR
ULTRASTRUCTURAL CHANGES