The effect of antioxidants on ozone-induced airway hyperresponsiveness in dogs

Journal Article · Sun Dec 01 04:00:00 EST 1991 · American Review of Respiratory Disease (New York); (United States)

DOI:https://doi.org/10.1164/ajrccm/144.6.1287· OSTI ID:5964512

Matsui, S; Jones, G L; Woolley, M J; Lane, C G; Gontovnick, L S; O'Byrne, P M ^[1]

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1. Department of Medicine, McMaster University, Hamilton, Ontario (Canada)

The role of oxygen radicals in causing ozone-induced airway hyperresponsiveness in dogs was examined by pretreating dogs with allopurinol and/or deferoxamine mesylate (desferal), which are inhibitors of oxygen radical generation, before ozone inhalation. Acetylcholine airway responsiveness was measured before and after either air or ozone inhalation (3 ppm for 20 min) on 5 experimental days separated by at least 2 wk. On each day, the dogs were pretreated intravenously with allopurinol (50 mg/kg) followed by inhaled desferal (1,000 mg inhalation) or with allopurinol followed by the diluent for desferal or with the diluent for allopurinol and desferal or with both diluents. The effect of ozone on acetylcholine airway responsiveness was expressed as the differences in the log-transformed preozone-postozone acetylcholine provocative concentrations. When dogs received both diluents or either treatment alone, ozone inhalation caused airway hyperresponsiveness. The mean log differences for the preozone-postozone acetylcholine provocative concentration were 0.804 (SEM, 0.17) for both diluents, 0.524 (SEM, 0.16) for allopurinol alone, and 0.407 (SEM, 0.22) for desferal alone. However, the combination of allopurinol and desferal significantly inhibited the development of ozone-induced airway hyperresponsiveness, the log difference being 0.195 (SEM, 0.11) (p less than 0.05), without inhibiting ozone-induced neutrophil influx into the airways. The results suggest that the production of oxygen radicals is important in the pathogenesis of ozone-induced airway hyperresponsiveness.

• OSTI ID: 5964512
• Journal Information: American Review of Respiratory Disease (New York); (United States), Journal Name: American Review of Respiratory Disease (New York); (United States) Vol. 144:6; ISSN ARDSB; ISSN 0003-0805
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIOXIDANTS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CHELATING AGENTS
DEFEROXAMINE
DOGS
DRUGS
ESTERS
INHALATION
INTAKE
LAVAGE
LUNGS
MAMMALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
OZONE
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADICALS
RESPIRATORY SYSTEM
SENSITIVITY
TOXICITY
VERTEBRATES