Analytical cytology applied to detection of prognostically important cytogenetic aberrations: Current status and future directions

Conference · Fri Jul 24 04:00:00 EDT 1987

OSTI ID:5963454

Gray, J W; Pinkel, D; Trask, B; van den Engh, G; Pallavicini, M; Fuscoe, J; Mullikin, J; van Dekken, H

This paper discusses the application of analytical cytology to the detection of clinically important chromosome abnormalities in human tumors. Flow cytometric measurements of DNA distributions have revealed that many human tumors have abnormal (usually elevated) DNA contents and that the occurrence of DNA abnormality may be diagnostically or prognostically important. However, DNA indices (ratio of tumor DNA content to normal DNA content) provide little information about the specific chromosome(s) involved in the DNA content abnormality. Fluorescence in situ hybridization with chromosome specific probes is suggested as a technique to facilitate detection of specific chromosome aneuploidy in interphase and metaphase human tumor cells. Fluorescence hybridization to nuclei on slides allows enumeration of brightly fluorescent nuclear domains as an estimate of the number of copies of the chromosome type for which the hybridization probe is specific. Fluorescence hybridization can also be made to nuclei in suspension. The fluorescence intensity can then be measured flow cytometrically as an indication of the number of chromosomes in each nucleus carrying the DNA sequence homologous to the probe. In addition, quantitative image analysis may be used to explore the position of chromosomes in interphase nuclei and to look for changes in the order that may eventually permit detection of clinicaly important conditions. 55 refs., 8 figs., 1 tab.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5963454
• Report Number(s): UCRL-97163; CONF-8704215-1; ON: DE88000750
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550600* -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANEUPLOIDY
CELL FLOW SYSTEMS
CELL TRANSFORMATIONS
CHROMOSOMAL ABERRATIONS
CHROMOSOME SORTING
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
EMISSION SPECTROSCOPY
FLUORESCENCE SPECTROSCOPY
MUTATIONS
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
PLOIDY
SPECTROSCOPY
USES