Identification of nuclear proteins that specifically bind to RNAs containing 5' splice sites
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Baylor College of Medicine, Houston, TX (USA)
Two polypeptides of 26 and 37 kDa (designated SPP-1 and SPP-2) were identified in in vitro splicing extracts by UV crosslinking to splicing precursor RNAs. Crosslinking of both polypeptides required a functional 5' splice site but was not dependent on sequences at the 3' end of the intron. Centrifugation of extract separated the two polypeptides from major U small nuclear ribonucleoproteins (snRNPs), including U1 snRNPs. Both polypeptides crosslinked to precursor RNAs containing 5' splice sites in the absence of U1 RNA. Complexes containing both polypeptides also contained U1 snRNPs, suggesting that SPP-1 and SPP-2 are a part of the functional spliceosome. We propose that SPP-1 and SPP-2 are factors that participate in the recognition of 5' splice sites.
- OSTI ID:
- 5963073
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 88:2; ISSN 0027-8424; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
ANIMALS
CENTRIFUGATION
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
ELECTROMAGNETIC RADIATION
FRACTIONATION
MAMMALS
MAN
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
POLYMERIZATION
PRIMATES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RNA PROCESSING
SEPARATION PROCESSES
ULTRACENTRIFUGATION
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
ANIMALS
CENTRIFUGATION
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
ELECTROMAGNETIC RADIATION
FRACTIONATION
MAMMALS
MAN
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PARASITES
POLYMERIZATION
PRIMATES
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RNA PROCESSING
SEPARATION PROCESSES
ULTRACENTRIFUGATION
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES