Hexose uptake in 7,12-dimethylbenz(a)anthracene-preexposed rat tracheal epithelial cells during the progression of neoplasia
Hexose uptake during the progression of neoplasia in rat tracheal epithelial cells was studied by measuring the uptake of 2-deoxy(/sup 3/H)glucose (2-dGlc) in nontumorigenic (C-18) and tumorigenic (T-8, 1000-WT) rat tracheal epithelial cell lines with varying degrees of cell association as well as in: (a) normal primary cell cultures (NPC) derived from explants of nonexposed tracheas; (b) selected primary cell cultures (SPC) generated from explants of 7,12-dimethylbenz(a)anthracene-treated tracheal implants; and (c) primary tumor cell cultures (TPC) derived from explants of 7,12-dimethylbenz(a)anthracene-induced tracheal carcinomas. The latter two groups represented cells from earlier and late states in the progression of neoplasia, respectively, and each displayed an in vitro growth advantage that allowed for their survival and growth in medium devoid of supplements of pyruvate and insulin. This property was used in this study to select the carcinogen-altered cells from neighboring normal cells. Uptake of 2-dGlc per ..mu..g of DNA was similar in subconfluent cultures of all cell lines. At confluency, uptake per ..mu..g of DNA was reduced markedly (>3-fold) in C-18 cells but it was reduced only 1.3-fold in T-8 cells and 1.6-fold in 1000-WT cells. Hexose uptake was further reduced in T-8 and 1000-WT cell cultures generated as outgrowths from explants of denuded tracheas bearing a reestablished epithelium from each cell line. Under these conditions, T-8 cells retained higher 2-dGlc uptake than did C-18, but uptake by 1000-WT was lower, indicating that tissue-like cell associations have a profound effect on hexose uptake in these epithelial cells. Results were generally similar when uptake was expressed per mg of protein although, in several instances, the interpretation of uptake data was affected by differences in the protein content between cultures. 46 references, 1 figure, 7 tables.
- Research Organization:
- Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5962932
- Journal Information:
- Cancer Res.; (United States), Vol. 44
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DIMETHYLBENZANTHRACENE
CARCINOGENESIS
HEXOSES
UPTAKE
TRACHEA
CARCINOMAS
CELL CULTURES
EPITHELIUM
EXPERIMENTAL DATA
GLUCOSE
RATS
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
ALDEHYDES
ANIMAL TISSUES
ANIMALS
AROMATICS
BODY
CARBOHYDRATES
CONDENSED AROMATICS
DATA
DISEASES
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
NEOPLASMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOGENESIS
RESPIRATORY SYSTEM
RODENTS
SACCHARIDES
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550901 - Pathology- Tracer Techniques