Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Brain Research Bulletin; (USA)

DOI:https://doi.org/10.1016/0361-9230(90)90186-4· OSTI ID:5962783

Dawson, V L; Dawson, T M; Wamsley, J K ^[1]

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1. Neuropsychiatric Research Institute, Fargo, ND (USA)

Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers (3H)hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change from control was observed in tyrosine hydroxylase or tryptophan hydroxylase activity; presynaptic neuronal markers for dopamine- and serotonin-containing neurons, respectively. The AF64A lesion resulted in a significant reduction of dopamine D2 receptors as evidenced by a decrease in (3H)sulpiride binding (42% reduction) and decrease of muscarinic non-M1 receptors as shown by a reduction in (3H)QNB binding in the presence of 100 nM pirenzepine (36% reduction). Saturation studies revealed that the change in (3H)sulpiride and (3H)QNB binding was due to a change in Bmax not Kd. Intrastriatal injection of AF64A failed to alter dopamine D1 or muscarinic M1 receptors labeled with (3H)SCH23390 and (3H)pirenzepine, respectively. In addition, no change in (3H)forskolin-labeled adenylate cyclase was observed. These results demonstrate that a subpopulation of muscarinic receptors (non-M1) are presynaptic on cholinergic interneurons (hence, autoreceptors), and a subpopulation of dopamine D2 receptors are postsynaptic on cholinergic interneurons. Furthermore, dopamine D1, muscarinic M1 and (3H)forskolin-labeled adenylate cyclase are not localized to striatal cholinergic interneurons.

• OSTI ID: 5962783
• Journal Information: Brain Research Bulletin; (USA), Journal Name: Brain Research Bulletin; (USA) Vol. 25:6; ISSN BRBUD; ISSN 0361-9230
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MARKERS
BODY
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CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHOLINE
CYCLASES
DOPAMINE
DRUGS
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HYDROXY COMPOUNDS
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ISOTOPE APPLICATIONS
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