Fibroblast growth factor-mediated proliferation of central nervous system precursors depends on endogenous production of insulin-like growth factor I

Journal Article · Fri Mar 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.88.6.2199· OSTI ID:5962514

Drago, J; Murphy, M; Carroll, S M; Harvey, R P; Bartlett, P F ^[1]

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1. Walter and Eliza Hall Institute of Medical Research, Royal Parade, Parkville, Victoria (Australia)

Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.

• OSTI ID: 5962514
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 88:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CENTRAL NERVOUS SYSTEM
CELL PROLIFERATION
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
AUTORADIOGRAPHY
DNA REPLICATION
EMBRYOS
EPITHELIUM
FIBROBLASTS
INHIBITION
INSULIN
MICE
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
KINETICS
MAMMALS
MITOGENS
NERVOUS SYSTEM
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques