Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism
- Michigan Technological Univ., Houghton (USA)
Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-(2-{sup 3}H)inositol or ({sup 32}Pi), extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-(2-{sup 3}H)inositol and ({sup 32}Pi)-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of ({sup 3}H)inositol prelabeled aleurone layers with GA{sub 3} showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.
- OSTI ID:
- 5962288
- Journal Information:
- Plant Physiology; (USA), Vol. 91:4; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHOSPHOLIPIDS
BIOCHEMICAL REACTION KINETICS
BARLEY
GIBBERELLIC ACID
INOSITOLS
PHOSPHORUS 32
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
ESTERS
GRASS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LACTONES
LIGHT NUCLEI
LILIOPSIDA
LIPIDS
MAGNOLIOPHYTA
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques