Phosphoinositides in barley aleurone layers and gibberellic acid-induced changes in metabolism

Journal Article · Thu Nov 30 23:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.91.4.1266· OSTI ID:5962288

Murthy, P P.N.; Renders, J M; Keranen, L M ^[1]

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1. Michigan Technological Univ., Houghton (USA)

Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-(2-{sup 3}H)inositol or ({sup 32}Pi), extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-(2-{sup 3}H)inositol and ({sup 32}Pi)-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of ({sup 3}H)inositol prelabeled aleurone layers with GA{sub 3} showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.

• OSTI ID: 5962288
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 91:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BARLEY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBOXYLIC ACIDS
CEREALS
DAYS LIVING RADIOISOTOPES
ESTERS
GIBBERELLIC ACID
GRASS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LACTONES
LIGHT NUCLEI
LILIOPSIDA
LIPIDS
MAGNOLIOPHYTA
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS