F-actin is intermolecularly crosslinked by N,N'-p-phenylene-dimaleimide through lysine-191 and cysteine-374

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.81.21.6599· OSTI ID:5955526

Elzinga, M; Phelan, J J

The bifunctional reagent N,N'-p-phenylene-dimaleimide (PDM) is being used in an attempt to measure distances between specific side chains in adjacent monomers within F-actin. (/sup 14/C)PDM was synthezised and was used to crosslink F-actin. Uncrosslinked actin was removed by gel filtration, and, from an arginine-specific tryptic digest of the covalently crosslinked dimers and higher oligomers, one radioactive crosslinked peptide was obtained in high yield. Amino acid composition and sequence analysis indicated that it comprises residues 184-196 of one monomer and 373-375 of an adjacent actin molecule, bridged by PDM through Cys-374 and Lys-191. Thus, these groups are shown to be 1.2-1.4 nm apart in adjacent actin monomers in F-actin. This information may be crucial in establishing the orientation of actin monomers within F-actin. 20 references, 3 figures, 2 tables.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5955526
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81:21; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTIN
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
CYSTEINE
DICARBOXYLIC ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSINE
MALEIC ACID
MAMMALS
MOLECULAR STRUCTURE
MONOMERS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYMERIZATION
PROTEINS
RABBITS
REAGENTS
THIOLS
TRACER TECHNIQUES
VERTEBRATES