Chemico/biological investigation of contaminated sediment from the Hamilton Harbour area of western Lake Ontario

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850220203· OSTI ID:5952768

Marvin, C H; Allan, L; McCarry, B E; Bryant, D W ^[1]

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1. McMaster Univ., Hamilton, Ontario (Canada)

Highly contaminated sediment from the Hamilton Harbour area of western Lake Ontario was examined using a bioassay-directed fractionation methodology. A sediment sample was extracted using a Soxhlet apparatus and the resulting extract was fractionated into compound classes using an alumina clean-up step and high performance liquid chromatographic techniques. The resulting fractions were subjected to bioassays using TA98- and TA100-like strains modified by the inclusion of genes for the activating enzymes nitroreductase and O-acetyl-transferase. The majority of the mutagenic activity displayed by the sample extract was found to be present in the fraction containing the polycyclic aromatic hydrocarbons (PAH). Extracts of the PAH-containing fraction displayed dramatically higher responses with the TA100 type strains with metabolic activation. Further separation of the PAH-containing fraction showed the majority of the biological activity coeluted with PAH having molecular masses of 276, 278, and 302 amu.

• OSTI ID: 5952768
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 22:2; ISSN EMMUEG; ISSN 0893-6692
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIOASSAY
CONTAMINATION
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
FRACTIONATION
GENES
GREAT LAKES
HYDROCARBONS
LAKE ONTARIO
LAKES
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGENESIS
ORGANIC COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
SALMONELLA
SALMONELLA TYPHIMURIUM
SEDIMENTS
SEPARATION PROCESSES
SURFACE WATERS