Conversion of monogalactosyldiacylglycerols to triacylglycerols in ozone-fumigated spinach leaves. [Spinacia oleracea L]
- National Institute for Environmental Studies, Ibaraki (Japan) Keio Univ., Tokyo (Japan) Univ. of Tokyo (Japan)
Molecular species and fatty acid distribution of triacylglycerol (TG) accumulated in spinach (Spinacia oleracea L.) leaves fumigated with ozone (0.5 microliter per liter) were compared with those of monogalactosyldiacylglyerol (MGDG). Analysis of positional distribution of the fatty acids in MGDG and the accumulated TG by the enzymatic digestion method showed that hexadecatrienoate (16:3) was restricted to sn-2 position of the glycerol backbone in both MGDG and TG, whereas {alpha}-linolenate (18:3) was preferentially located at sn-1 position in MGDG, and sn-1 and/or sn-3 positions in TG, suggesting that 1,2-diacylglycerol moieties of MGDG are the direct precursor of TG in ozone-fumigated leaves. Further analysis of TG molecular species by argentation chromatography and mass spectrometry showed that TG increased with ozone fumigation consisted of approximately an equal molar ratio of sn-1,3-18:3-2-16:3 and sn-1,2,3-18:3. Because the molecular species of MGDG in spinach leaves is composed of a similar molar ratio of sn-1-18:3-2-16:3 and sn-1,2-18:3, we conducted that MGDG was converted to 1,2-diacylglycerol and acylated with 18:3 to TG in ozone-fumigated spinach leaves.
- OSTI ID:
- 5950519
- Journal Information:
- Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:2; ISSN 0032-0889; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
FOOD
KINETICS
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OZONE
PHYSIOLOGY
PLANTS
REACTION KINETICS
SPINACH
TOXICITY
VEGETABLES