Kinetics of hydrogen consumption by methanogenic consortia and cultures of hydrogen-consuming anaerobes. [Methanospirillum PM1, M. hungatei JF-1, Methanosarcina barkeri MS, Methanobacterium PM2, Desulfovibrio G11 and PS1]

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1982

OSTI ID:5950373

Robinson, J A

Hydrogen plays a central role in the breakdown of organic matter in anaerobic habitats, influencing the nature of the fermentation end products and possibly, rates at which the initial substrates are degraded. The kinetics were examined for H/sub 2/ consumption by samples from natural anaerobic habitats, pure cultures of H/sub 2/-consuming anaerobes, and co-cultures comprised of methanogenic and sulfate-reducing bacteria. These kinetic studies were performed using a gas-recirculation system that allowed precise measurements of gaseous phase H/sub 2/ and CH/sub 4/. Uptake and growth kinetic parameters were estimated for the natural samples and suspensions of H/sub 2/-consumers by fitting H/sub 2/ depletion (progress curve) data to integrated forms of Michaelis-Menten and Monod equations. Samples included eutrophic lake sediments, anaerobic digestor sludge, and rumen fluid. The bacteria studied were methanospirillum PM1, Methanosarcina barkeri MS, Methanospirillum hungatei JF-1, Methanohbacterium PM2, and Desulfovibrio strains G11 and PS1.

• OSTI ID: 5950373
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090122* -- Hydrocarbon Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKANES
ANAEROBIC DIGESTION
ANIMALS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOCONVERSION
BIOREACTORS
BIOSYNTHESIS
DIGESTION
ELEMENTS
EQUATIONS
GAS ANALYSIS
GROWTH
HYDROCARBONS
HYDROGEN
KINETICS
LAKES
MAMMALS
MANAGEMENT
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
PROCESSING
REACTION KINETICS
RUMINANTS
SEDIMENTS
SLUDGES
SULFATE-REDUCING BACTERIA
SURFACE WATERS
SYNTHESIS
UPTAKE
VERTEBRATES
WASTE MANAGEMENT
WASTE PROCESSING