Xylem-to-phloem transfer of organic nitrogen in young soybean plants

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.3.797· OSTI ID:5946343

Da Silva, M C; Shelp, B J ^[1]

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1. Univ. of Guelph, Ontario (Canada)

Xylem-to-phloem transfer in young vegetative soybean (Glycine max (L.) Merr.) plants (V4 stage) was identified as the difference in the distribution of ({sup 14}C)inulin, a xylem marker, and ({sup 14}C)aminoisobutyric acid (AIB), a synthetic amino acid, fed via the transpiration stream. Since ({sup 14}C)AIB was retained in the stem to some extent, whereas ({sup 14}C)inulin was not, the distribution of these marker compounds in each leaf was expressed as a percentage of the total ({sup 14}C) radioactivity recovered in the foliage. The developing third trifoliolate was a consistent and reliable indicator of xylem-to-phloem transfer. The phloem stream provided to the developing trifoliolate up to fourfold the relative proportion of solute received from the xylem stream; this was markedly reduced by increased light intensity and consequently water flow through the xylem. Evidence from heat girdling experiments is discussed with respect to the vascular anatomy of the soybean plant, and interpreted to suggest that direct xylem-to-phloem transfer in the stem, in the region of the second node, accounted for about one-half of the AIB supplied to the developing trifoliolate, with the remainder being provided from the second trifoliolate. Since AIB is not metabolized it seems likely that rapid transfer within the second trifoliolate occurred as direct veinal transfer rather than indirect cycling through the mesophyll. This study confirmed that xylem-to-phloem transfer plays a major role in the partitioning of nitrogen for early leaf development.

• OSTI ID: 5946343
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 92:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
CARBON 14 COMPOUNDS
ELEMENTS
GLYCINE HISPIDA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MATERIALS
METABOLISM
NITROGEN
NONMETALS
PLANT SAP
PLANTS
TRACER TECHNIQUES
TRANSPIRATION