Photosynthetic carbon metabolism in photoautotrophic cell suspension cultures grown at low and high CO sub 2

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.91.4.1512· OSTI ID:5945906

Roeske, C A; Widholm, J M ^[1]; Ogren, W L ^[2]

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1. Univ. of Illinois at Urbana-Champaign (USA)
2. Department of Agriculture, Urbana, IL (USA)

Photosynthetic carbon metabolism was characterized in four photoautotrophic cell suspension cultures. There was no apparent difference between two soybeans (Glycine max) and one cotton (Gossypium hirsutum) cell line which required 5% CO{sub 2} for growth, and a unique cotton cell line that grows at ambient CO{sub 2} (660 microliters per liter). Photosynthetic characteristics in all four lines were more like C{sub 3} mesophyll leaf cells than the cell suspension cultures previously studied. The pattern of {sup 14}C-labeling reflected the high ratio of ribulosebisphosphate carboxylase to phosphoenolpyruvate carboxylase activity and showed that CO{sub 2} fixation occurred primarily by the C{sub 3} pathway. Photorespiration occurred at 330 microliters per liter CO{sub 2}, 21% O{sub 2} as indicated by the synthesis of high levels of {sup 14}C-labeled glycine and serine in a pulse-chase experiment and by oxygen inhibition of CO{sub 2} fixation. Short-term CO{sub 2} fixation in the presence and absence of carbonic anhydrase showed CO{sub 2}, not HCO{sub 3}{sup {minus}}, to be the main source of inorganic carbon taken up by the low CO{sub 2}-requiring cotton cells. The cells did not have a CO{sub 2}-concentrating mechanism as indicated by silicone oil centrifugation experiments. Carbonic anhydrase was absent in the low CO{sub 2}-requiring cotton cells, present in the high CO{sub 2}-requiring soybean cell lines, and absent in other high CO{sub 2} cell lines examined. Thus, the presence of carbonic anhydrase is not an essential requirement for photoautotrophy in cell suspension cultures which grow at either high or low CO{sub 2} concentrations.

• OSTI ID: 5945906
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 91:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540110
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CULTURES
CHALCOGENIDES
CHEMICAL REACTIONS
COTTON PLANTS
GLYCINE HISPIDA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
SYNTHESIS
TRACER TECHNIQUES