IL-3 specifically inhibits GM-CSF binding to the higher affinity receptor

Journal Article · Fri Feb 01 00:00:00 EST 1991 · Journal of Cellular Physiology; (USA)

DOI:https://doi.org/10.1002/jcp.1041460209· OSTI ID:5945756

Taketazu, F; Chiba, S; Shibuya, K; Kuwaki, T; Tsumura, H; Miyazono, K; Miyagawa, K; Takaku, F ^[1]

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1. Univ. of Tokyo (Japan)

The inhibition of binding between human granulocyte-macrophage colony-stimulating factor (GM-CSF) and its receptor by human interleukin-3 (IL-3) was observed in myelogenous leukemia cell line KG-1 which bore the receptors both for GM-CSF and IL-3. In contrast, this phenomenon was not observed in histiocytic lymphoma cell line U-937 or in gastric carcinoma cell line KATO III, both of which have apparent GM-CSF receptor but an undetectable IL-3 receptor. In KG-1 cells, the cross-inhibition was preferentially observed when the binding of GM-CSF was performed under the high-affinity binding condition; i.e., a low concentration of 125I-GM-CSF was incubated. Scatchard analysis of 125I-GM-CSF binding to KG-1 cells in the absence and in the presence of unlabeled IL-3 demonstrated that IL-3 inhibited GM-CSF binding to the higher-affinity component of GM-CSF receptor on KG-1 cells. Moreover, a chemical cross-linking study has revealed that the cross-inhibition of the GM-CSF binding observed in KG-1 cells is specific for the beta-chain, Mr 135,000 binding protein which has been identified as a component forming the high-affinity GM-CSF receptor existing specifically on hemopoietic cells.

• OSTI ID: 5945756
• Journal Information: Journal of Cellular Physiology; (USA), Vol. 146:2; ISSN 0021-9541
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
LYMPHOKINES
AFFINITY
CARCINOMAS
COLONY FORMATION
INHIBITION
IODINE 125
LEUKEMIA
LYMPHOMAS
MACROPHAGES
MAN
REAGENTS
RECEPTORS
TRACER TECHNIQUES
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques