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Macrophage-induced cytostasis: kinetic analysis of bromodeoxyuridine-pulsed cells

Journal Article · · Cytometry (Baltimore); (United States)
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The effect of tumoricidal macrophages on the cell cycle progression of six different cell lines was studied using an anti-bromodeoxyuridine (BrdUrd) monoclonal antibody to follow the traverse of BrdUrd-labeled cells. Exponentially growing cultured mammalian cells, from six different cell lines, were prepulsed with BrdUrd before exposure to tumoricidal macrophages. The cultured cells were then analyzed as a function of time for DNA content (by propidium iodide staining) and for BrdUrd incorporation (using a fluoresceini-sothiocyanate (FITC)-conjugated anti-BrdUrd monoclonal antibody). The position of the cells in cycle and the progression of the BrdUrd-labeled cohort was followed using flow cytometry. The cell lines examined were: Colon 26; BALB/c-3T3, ST3T3 (a spontaneously transformed, tumorigenic clone of 3T3), WCHE5 (a clone of whole Chinese hamster embryo cells), RIF (a radiation-induced fibrosarcoma), and A101D (a human melanoma). The bivariate distributions showed that for all six cell lines the BrdUrd-labeled cohort in the control cultures progressed around the cell cycle during the first 12 h of culture, as the cells exponentially increased. In contrast, when each cell line was incubated with tumoricidal macrophages, the BrdUrd-labeled cohort did not progress through cell cycle but remained in S phase throughout the 12-h culture period. There was also no evidence for progression of cells out of G/sub 1/. The data show that cells were arrested in every phase of cell cycle. This study suggests that cytostasis, as manifested by the termination of progression in all phases of the cell cycle, is a universal phenomenon induced by tumoricidal macrophages. 20 references, 4 figures.

Research Organization:
Los Alamos National Lab., NM
OSTI ID:
5941832
Journal Information:
Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 6; ISSN CYTOD
Country of Publication:
United States
Language:
English

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Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIMETABOLITES
AZINES
BIOASSAY
BIOLOGICAL FUNCTIONS
BROMOURACILS
BUDR
CELL CULTURES
CELL CYCLE
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DRUGS
FUNCTIONS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MACROPHAGES
MAMMALS
MAN
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
PRIMATES
PYRIMIDINES
RADIOINDUCTION
RIBOSIDES
RODENTS
SARCOMAS
SOMATIC CELLS
TOXICITY
URACILS
VERTEBRATES