Studies of the uptake of nitrate in barley. II. Energetics. [Hordeum vulgare L]

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.4.1585· OSTI ID:5941561

Glass, A D.M.; Siddiqi, M Y; Ruth, T J; Rufty, Jr, T W ^[1]

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1. Univ. of British Columbia, Vancouver (Canada) North Carolina State Univ., Raleigh (USA)

Q{sub 10} values for {sup 13}NO{sub 3}{sup {minus}} influx were determined in uninduced (NO{sub 3}{sup {minus}}-starved) and induced (NO{sub 3}{sup {minus}}-pretreated) roots of barley (Hordeum vulgare L.) plants at various concentrations of external NO{sub 3}{sup {minus}} ((NO{sub 3}{sup {minus}}){sub 0}). At 0.02 mole per cubic meter (NO{sub 3}{sup {minus}}){sub 0}, Q{sub 10} values for influx were from 3 to 4 between 5 and 10{degree}C. As (NO{sub 3}{sup {minus}}){sub 0} increased Q{sub 10} values decreased, reaching values of 1.2 and 2.0, respectively, at 20 moles per cubic meter in uninduced and induced plants. The metabolic dependence of {sup 13}NO{sub 3}{sup {minus}} influx at low and high (NO{sub 3}{sup {minus}}){sub 0} (0.1 and 20.0 moles per cubic meter, respectively) in uninduced and induced plants was probed by the use of various inhibitors. These experiments confirmed the findings of the Q{sub 10} studies, demonstrating that at low (NO{sub 3}{sup {minus}}){sub 0} {sup 13}NO{sub 3}{sup {minus}} influx was extremely sensitive to metabolic inhibition. By contrast, at high (NO{sub 3}{sup {minus}}){sub 0}, influx was relatively insensitive to the presence of inhibitors.

• OSTI ID: 5941561
• Journal Information: Plant Physiology; (USA), Vol. 93:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NITRATES
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INHIBITION
NITROGEN 13
PHYSIOLOGY
THERMODYNAMICS
TRACER TECHNIQUES
ABSORPTION
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
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ISOTOPES
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NITROGEN COMPOUNDS
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NUCLEI
ODD-EVEN NUCLEI
OXYGEN COMPOUNDS
RADIOISOTOPES
UPTAKE
551001* - Physiological Systems- Tracer Techniques