Pulmonary fibrosis in experimental actue respiratory disease. [Rats]
Changes in collagen metabolism were examined in 3 models of acute respiratory disease in rats. Fibrotic changes in the lungs of rats were provoked by exposing them to paraquat (intraperitoneal), ozone (inhaled), or bleomycin (intratracheally injected). After an interval sufficient to allow histologically discernible fibrosis to occur (6 to 7 days), lungs were removed from the rats, and apparent collagen synthesis rates were determined with cultured lung minces incubated in medium containing 3H-proline. Portions of the 3H-proline-labeled lung minces were then used for quantifying ratios of Type I to Type III collagen. There was no change in Type I/Type III collagen for total unlabeled collagen, nor was there any detectable increase of total collagen per lung after 1 week. We conclude that an early event in experimental acute respiratory disease is a marked increase in the relative synthesis of Type I collagen; this shift occurs before there is observable increased accumulation of collagen in the lung.
- OSTI ID:
- 5940342
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Vol. 123:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BLEOMYCIN
BIOLOGICAL EFFECTS
COLLAGEN
BIOSYNTHESIS
CHROMATOGRAPHY
ELECTROPHORESIS
INSECTICIDES
OZONE
BIOLOGICAL MODELS
FIBROSIS
LUNGS
RATS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BODY
DRUGS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PESTICIDES
PROTEINS
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)
550200 - Biochemistry