Immunolymphoscintigraphy with Indium-111 labeled T101 monoclonal antibody

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:5938610

Keenan, A M; Weinstein, J N; Carrasquillo, J A; Reynolds, J C; Perentesis, P; Foon, K A; Mulshine, J L; Bunn, P A; Larson, S M

The authors have demonstrated specific modal and cutaneous uptake of In-111 T101 (a pan T-cell monoclonal antibody) after intravenous (IV) infusion into patients with cutaneous T-cell lymphoma (CTCL); however since animal studies have shown more efficient lymph node (LN) uptake after subcutaneous (SC) injection, this technique was applied to several patients with CTCL. Serial gamma camera images were obtained after SC injection of 500 ..mu..Ci In-111 T101 (100 ..mu..g, 500 ..mu..g or 1 mg) into the webs between the toes. Within minutes, lymphatic vessels in the legs as well as femoral, inguinal and iliac LN were visualized in all patients. Computer-analysis of serial images demonstrated that LN counts reached maximum at 24 hr and remained stable through 96 hr. At 24 hrs, femoral and inguinal LN collectively contained 18% to 51% of the injected dose (ID), and the injection site retained from 23% to 51% of the ID. In contrast to the results of IV injection, the liver, spleen, marrow and supradiaphragmatic LN (cervical, hilar, and axillary) contained much less radiolabel than did subdiaphragmatic LN. Inguinal lN biopsies showed up to 2% of the ID per gram. Low (100 ..mu..g) dose administration resulted in strong uptake in inguinal and iliac LN with weak uptake in periaortic LN, whereas 500 ..mu..g and above produced strong uptake in all subdiaphragmatic LN. These findings suggest highly efficient binding in LN proximal to the injection site, followed by more distant uptake as the proximal sites approach saturation, and demonstrate that lymphatic delivery of monoclonal antibodies can be used to target cells in human LN.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5938610
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
COUNTING TECHNIQUES
DATA ANALYSIS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOMAS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SUBCUTANEOUS INJECTION
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE
USES