Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Assessment of preferential cleavage of an actively transcribed retroviral hybrid gene in murine cells by deoxyribonuclease I, bleomycin, neocarzinostatin, or ionizing radiation

Journal Article · · Biochemistry; (United States)
DOI:https://doi.org/10.1021/bi00391a029· OSTI ID:5934727
; ; ; ;

Preferential cleavage induced by bleomycin, neocarzinostatin, or ionizing radiation in a transcribed cellular gene was evaluated through comparisons with deoxyribonuclease I. The glucocorticoid-inducible LTL gene previously described served as the specific DNA target. A Southern blot analysis was used to specifically assess cleavage of the LTL gene in nuclei isolated from cells either treated or untreated with the synthetic glucocorticoid dexamethasone. Hypersensitivity of the gene to bleomycin or neocarzinostatin, which paralleled deoxyribonuclease I hypersensitivity, was evident only in nuclei isolated from dexamethasone-treated cells. Like deoxyribonuclease I, sites of dexamethasone-inducible drug hypersensitivity were coincident with the binding region for the glucocorticoid receptor found within the regulatory sequences of the LTL gene. In contrast, no hypersensitivity to ionizing radiation was evident. Although bleomycin and neocarzinostatin showed qualitatively similar preferences for the threshold LTL gene, quantitative evaluations of damage to total cellular DNA by filter elution showed that the relative specificity of bleomycin for the hypersensitive region was much less than that of either deoxyribonuclease I or neocarzinostatin.

Research Organization:
Roswell Park Memorial Institute, Buffalo, NY
OSTI ID:
5934727
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:17; ISSN BICHA
Country of Publication:
United States
Language:
English

Similar Records

Effect of apurinic/apyrimidinic endonucleases and polyamines on DNA treated with bleomycin and neocarzinostatin: specific formation and cleavage of closely opposed lesions in complementary strands
Journal Article · Tue May 17 00:00:00 EDT 1988 · Biochemistry; (United States) · OSTI ID:7024779
Glucocorticoid inhibition of cellular proliferation in rat hepatoma cell lines
Thesis/Dissertation · Wed Dec 31 23:00:00 EST 1986 · OSTI ID:5564198
Glucocorticoid coordinate regulation of type I procollagen gene expression and procollagen DNA-binding proteins in chick skin fibroblasts
Journal Article · Tue Apr 19 00:00:00 EDT 1988 · Biochemistry; (United States) · OSTI ID:7253561

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLEOMYCIN
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DNA
DNA-ASE
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMATIC HYDROLYSIS
ENZYMES
ESTERASES
GENES
HYDROLASES
HYDROLYSIS
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NEOCARCINOSTATIN
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOMIMETIC DRUGS
SOLVOLYSIS
VIRUSES
X RADIATION