Purification and characterization of an. alpha. -bungarotoxin receptor that forms a functional nicotinic channel

Journal Article · Mon Apr 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.8.3258· OSTI ID:5934269

Gotti, C; Ogando, A E; Moretti, M; Clementi, F ^[1]; Hanke, W; Schlue, R ^[2]

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1. Univ. of Milan (Italy)
2. Univ. of Duesseldorf (West Germany)

Neither the structure nor the function of {alpha}-bungarotoxin ({alpha}Bgtx) binding molecules in the nervous system have yet been completely defined, although it is known that some of these molecules are related to cation channels and some are not. Using an improved method of affinity chromatography, the authors have isolated a toxin binding molecule from chicken optic lobe that contains at least three subunits with apparent M{sub r} values of 52,000, 57,000, and 67,000. The M{sub r} 57,000 subunit binds {alpha}Bgtx receptors of human neuroblastoma cells, fetal calf muscle, and chicken optic lobe but not by antibodies raised against Torpedo acetylcholine receptor, the serum of myasthenic patients, or monoclonal antibody 35. {sup 125}I-labeled {alpha}Bgtx binding to the isolated receptor is blocked, with the same potency, by nicotinic agonists and antagonists, such as nicotine, neuronal bungarotoxin and, d-tubocurarine. When reconstituted in a planar lipid bilayer, the purified {alpha}Bgtx receptor forms cationic channels with a conductance of 50 pS. These channels are activated in a dose-dependent manner by carbamylcholine and blocked by d-tubocurarine.

• OSTI ID: 5934269
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:8; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMIDES
ANTIBODIES
ANTIGENS
AZINES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
MEMBRANE PORES
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NICOTINAMIDE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORS
PROTEINS
PURIFICATION
PYRIDINES
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SODIUM COMPOUNDS
SODIUM IODIDES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS