Influence of bacterial toxins on the GTPase activity of transducin from bovine retinal rod outer segments
The action of cholera toxin, capable of ADP-ribosylation of the activator N/sub s/ protein, and pertussis toxin, capable of ADP-ribosylation of the inhibitor N/sub i/ protein of the adenylate cyclase complex, on transducin, the GTP-binding protein of the rod outer segments of the retina, was investigated. It was shown that under the action of pertussis and cholera toxins, the GTPase activity of transducin is inhibited. Pertussin toxin inhibits the GTPase of native retinal rod outer segments by 30-40%, while GTPase of homogeneous transducin produces a 70-80% inhibition. The action of toxins on transducin depends on the presence and nature of the guanylic nucleotide with which incubation is performed. On the basis of the data obtained it is suggested that pertussis toxin interacts with pretransducin and with the transducin-GDP complex, while cholera toxin ADP-ribosylates the transducin-GTP complex and does not act on transducin lacking GTP.
- Research Organization:
- Institute of Experimental Cardiology, Moscow, USSR
- OSTI ID:
- 5934062
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:11; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CATTLE
CHEMICAL REACTIONS
CHEMISTRY
CHOLERA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DISEASES
DOMESTIC ANIMALS
ELECTROPHORESIS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EYES
FACE
GUANOSINE
HEAD
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
INFECTIOUS DISEASES
INHIBITION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MAMMALS
MATERIALS
METABOLIC ACTIVATION
MICROORGANISMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
PURINES
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RETINA
RIBOSIDES
RUMINANTS
SENSE ORGANS
SOLVOLYSIS
SUBSTRATES
TOXIC MATERIALS
TOXINS
VERTEBRATES