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Use of (2-/sup 14/C)mevalonate and saponin-bound (/sup 14/C)-3-hydroxy-3-methylglutaric acid for the biosynthesis of terpenoids in leaves of Dioscorea deltoidea

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5934047
; ;

After the introduction of (2-/sup 14/C)acetate into leaves of Dioscorea deltoidea, a radioactive furonanalog of deltafolin - protodeltofolin, containing two-thirds of the label in the 3-hydroxy-3-methylglutaryl portion - was isolated from them. Radioactive ..beta..-careotene and sterols were isolated from cut young leaves of Dioscorea 24 h after the introduction of (/sup 14/C) protodeltofolin into them, using chromatography on a column of silica gel and precipitation of sterols in the form of digitonins for this purpose. The incorporation of radioactivity from (/sup 14/C)-3-hydroxy-3-methyl-glutaric acid, bound in the form of a saponin, and ..beta..-carotene came to 0.18-0.80%, while incorporation into sterols came to 0.07-2.86% of the radioactivity of the alcohol extract. Thereby it was shown that 3-hydroxyl-3-methylglutaric acid, bound in the form of the saponin, can be used to form terpenoids in Dioscorea leaves. It was suggested that the binding of hydroxymethylglutaric acid to saponin represents one of the mechanisms of regulation of the rate of terpenoid biosynthesis in Dioscorea leaves.

Research Organization:
A.N. Bakh Institute of Biochemistry, Moscow, USSR
OSTI ID:
5934047
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CAROTENOIDS
CHEMISTRY
CHROMATOGRAPHY
DICARBOXYLIC ACIDS
ESTERS
GLUTARIC ACID
GLYCOSIDES
HYDROCARBONS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEAVES
MEVALONIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
PIGMENTS
RADIORECEPTOR ASSAY
REACTION KINETICS
SAPONINS
SEPARATION PROCESSES
STEROIDS
STEROLS
SYNTHESIS
TERPENES
TRACER TECHNIQUES
UPTAKE