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Peculiarities of the inhibition of the pyruvate dehydrogenase complex by thiamine thiazolone diphosphate in vitro and in intact mitochondria

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5933645
; ; ;
Thiamine thiazolone diphosphate (TTPP) possesses the ability to penetrate through the mitochondrial membrane and inhibit the pyruvate dehydrogenase complex in intact mitochondria, TTPP inhibits the activity of the complex of animal origin according to a mixed type (K/sub i/ 5 x 10/sup -8/ M) and yeast pyruvate decarboxylase according to a competitive type (K/sub i/ 5 x 10/sup -6/ M) with respect to thiamine diphosphate (TPP). Decarboxylation of pyruvate in intact and lysed rat liver and brain mitochondria is inhibited in the presence of TTPP significantly more weakly than the total activity of the pyruvate dehydrogenase complex, determined according to the formation of acetyl-CoA. It is suggested that TTPP, as an analog of the transition state, acts only in dehydrogenase reactions but not at the stage of simple decarboxylation of pyruvate.
OSTI ID:
5933645
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:1; ISSN BIORA
Country of Publication:
United States
Language:
English

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550201 -- Biochemistry-- Tracer Techniques
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59 BASIC BIOLOGICAL SCIENCES
AMINES
AZINES
AZOLES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
CHEMISTRY
COMPLEXES
COMPLEXOMETRY
COUNTING TECHNIQUES
CYTOCHEMISTRY
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
KETO ACIDS
LABELLED COMPOUNDS
LABELLING
MEMBRANE TRANSPORT
MEMBRANES
MITOCHONDRIA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PYRIMIDINES
PYRUVIC ACID
RADIOACTIVITY
RADIOENZYMATIC ASSAY
SCINTILLATION COUNTING
THIAMINE
THIAZOLES
VITAMIN B GROUP
VITAMINS