Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA

Journal Article · Mon Jul 01 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.13.5744· OSTI ID:5933083

Campbell, C; Gulati, R; Nandi, A K; Kucherlapati, R S ^[1]; Floy, K; Hieter, P ^[2]

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1. Albert Einstein College of Medicine, Bronx, NY (United States)
2. Johns Hopkins Univ. School of Medicine, Baltimore, MD (United States)

The authors have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end of a sequence that is uniquely represented on the YAC and at the other end of a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.

• OSTI ID: 5933083
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:13; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA
ELECTROPHORESIS
EUMYCOTA
FUNGI
GENE RECOMBINATION
HETEROCHROMOSOMES
HUMAN X CHROMOSOME
MAMMALS
MAN
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
PRIMATES
RFLPS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
VERTEBRATES
X CHROMOSOME
YEASTS