Slow-growth phenotype of transgenic tomato expressing apoplastic invertase

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.95.2.420· OSTI ID:5930932

Dickinson, C D; Altabella, T; Chrispeels, M J ^[1]

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1. Univ. of California, San Diego, La Jolla (United States)

The growth of transgenic tomato (Lycopersicon esculentum) plants that express in their apoplast yeast invertase under the control of the cauliflower mosaic virus 35S promoter is severely inhibited. The higher the level of invertase, the greater the inhibition of growth. A second phenotypic characteristic of these transgenic plants is the development of yellow and necrotic spots on the leaves, and leaf curling. Again the severity of the symptoms is correlated with the level of invertase. These symptoms do not develop in shaded leaves indicating the need for photosynthesis. Keeping the plants in the dark for a prolonged period (24 hours) results in the disappearance of leaf starch from the control plants, but not from the plants with apoplastic invertase. These results are consistent with the interpretation that apoplastic invertase prevents photosynthate export from source leaves and that phloem loading includes an apoplastic step.

• OSTI ID: 5930932
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 95:2; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CHEMICAL REACTIONS
ENZYMES
FOOD
FRUITS
GENES
GENETIC ENGINEERING
GLYCOSYL HYDROLASES
GROWTH
HYDROLASES
INHIBITION
ORGANIC COMPOUNDS
PHENOTYPE
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHYSIOLOGY
PLANT GROWTH
PROTEINS
SYNTHESIS
TOMATOES