Isolation and measurement of /sup 15/N/sub 2/ from respiratory gases of animals administered /sup 15/N-labeled substances

Journal Article · Wed Jul 01 00:00:00 EDT 1981 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398109530046· OSTI ID:5926960

Springer, D L; Reed, D J; Dost, F N

A method is described for collection of metabolic /sup 15/N/sub 2/ from in vitro preparations or intact rats administered /sup 15/N-containing compounds. The method enables routine collection and mass spectrometric measurement of as little as 10 ..mu..mol /sup 15/N/sub 2/ respired by a rat over a 24-h period. A device is described that includes either an animal chamber or a tissue reaction vessel in a closed recycling atmosphere, with automatic O/sub 2/ replenishment and removal of CO/sub 2/ and water. It is capable of sustaining moderate vacuum and is coupled to a high-vacuum manifold designed to process the contained atmosphere and respiratory gases. The starting atmosphere is an 80:20 mix of sulfur hexafluoride and O/sub 2/. Recovery of /sup 15/N/sub 2/ gas from the system without an animal present was 101.3 +/- 5.75%. When /sup 15/N/sub 2/ gas was very slowly infused iv into an animal, recovery was 89.1 +/- 5.38%. Use of the method in studies of the fate of (/sup 15/N)hydrazine in rats indicated that about 15% of the administered hydrazine is rapidly converted to /sup 15/N/sub 2/, followed by slower conversion of an additional 7-10% over the next several hours.

• Research Organization: Oregon State Univ., Corvallis
• OSTI ID: 5926960
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 8:1-2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HYDRAZINE
METABOLISM
MEASURING METHODS
NITROGEN COMPOUNDS
MASS SPECTROSCOPY
EVALUATED DATA
EXPERIMENTAL DATA
NITROGEN 15
QUANTITATIVE CHEMICAL ANALYSIS
RATS
RESPIRATION
TRACER TECHNIQUES
ANIMALS
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DATA
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NITROGEN ISOTOPES
NUCLEI
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ODD-EVEN NUCLEI
RODENTS
SPECTROSCOPY
STABLE ISOTOPES
VERTEBRATES
550500* - Metabolism
550501 - Metabolism- Tracer Techniques