X-ray photoelectron spectroscopy surface analysis of aluminum ion stress in barley roots. [Hordeum vulgare]

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.2.578· OSTI ID:5922378

Millard, M M; Foy, C D; Coradetti, C A; Reinsel, M D ^[1]

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1. Department of Agriculture, Beltsville, MD (USA)

X-ray photoelectron spectroscopy (XPS) has been used to analyze root surface changes when Dayton barley (Hordeum vulgare) (Al tolerant) and Kearney barley (Al sensitive) seedlings were grown in nutrient solution in the presence and absence of 37.0 micromolar Al. The electron spectra from root surfaces contained strong lines in order of decreasing intensity from organic forms of carbon, oxygen, and nitrogen and weak lines due to inorganic elements in the form of anions and cations on the surface. The surface composition of root tips from Kearney was C, 65.6%; 0, 26.8%; N, 4.4% and tips from Dayton was C, 72.7%; O, 23.6%; N, 1.9%, grown in the absence of aluminum. Electron lines characteristic of nitrate, potassium, chloride, phosphate were also present in the spectra from those roots. Dayton roots grown in the presence of 37.0 micromolar aluminum contained 2.1% aluminum while Kearney contained 1.3% aluminum. The ratio of aluminum to phosphate was close to 1.0. Dayton roots usually contained twice as much aluminum phosphate in the surface region as Kearney. Dayton may be less susceptible to Al toxic effects by accumulation of aluminum phosphate on the root surface which then acts as a barrier to the transport of aluminum into the interior of the roots.

• OSTI ID: 5922378
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 93:2; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
BARLEY
BIOLOGICAL STRESS
CEREALS
CHEMICAL COMPOSITION
COMPARATIVE EVALUATIONS
ELEMENTS
GRASS
GROWTH
LILIOPSIDA
MAGNOLIOPHYTA
MEASURING METHODS
METALS
PHYSIOLOGY
PLANT GROWTH
PLANTS
ROOTS
SENSITIVITY
TOLERANCE
TOXICITY