In vitro oxidation of indoleacetic acid by soluble auxin-oxidases and peroxidases from maize roots. [Zea mays L]

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.2.485· OSTI ID:5915073

Beffa, R; Martin, H V; Pilet, P E ^[1]

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1. Institute of Plant Biology and Physiology of the University, Lausanne (Switzerland)

Soluble auxin-oxidases were extracted from Zea mays L. cv LG11 apical root segments and partially separated from peroxidases (EC 1.11.1.7) by size-exclusion chromatography. Auxin-oxidases were resolved into one main peak corresponding to a molecular mass of 32.5 kilodaltons and a minor peak at 54.5 kilodaltons. Peroxidases were separated into at least four peaks, with molecular masses from 32.5 to 78 kilodaltons. In vitro activity of indoleacetic acid-oxidases was dependent on the presence of MnCl{sub 2} and p-coumaric acid. Compound(s) present in the crude extract and several synthetic auxin transport inhibitors (including 2,3,5-triiodobenzoic acid and N-1-naphthylphthalamic acid) inhibited auxin-oxidase activity, but had no effect on peroxidases. The products resulting from the in vitro enzymatic oxidation of ({sup 3}H)indoleacetic acid were separated by HPLC and the major metabolite was found to cochromatograph with indol-3yl-methanol.

• OSTI ID: 5915073
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:2; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUXINS
BIOCHEMICAL REACTION KINETICS
CEREALS
CHEMICAL REACTIONS
ENZYMES
GRASS
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
OXIDATION
OXIDOREDUCTASES
PHYSIOLOGY
PLANT GROWTH REGULATORS
PLANTS
REACTION KINETICS
ROOTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS