Calcium fluxes across the plasma membrane of Commelina communis L. assayed in a cell-free system

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.3.940· OSTI ID:5914772

Siebers, B; Graef, P; Weiler, E W ^[1]

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1. Ruhr-Universitaet Bochum (West Germany)

The inside-out fraction of plasma membrane-rich vesicles prepared from leaves of Commelina communis L. by aqueous two-phase partitioning was loaded with {sup 45}Ca{sup 2+} through the action of the plasma membrane Ca{sup 2+}-ATPase. Results suggest the presence of a Ca{sup 2+} channel in the plasma membrane of C. communis. The channel is obtained in a Ca{sup 2+}-inactivated state after preparation and Ca{sup 2+}-loading of the vesicles. The inactivation is removed by TFP (trifluoperazine) or W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide), presumably due to the Ca{sup 2+}-mobilizing effect of these compounds. The activated Ca{sup 2+} channel is La{sup 3+} sensitive and, in the cell, would allow for passage of Ca{sup 2+} into the cell. The possibility that TFP or W-7 act independent of CM, or through CM tightly associated with the plasma membrane, is discussed.

• OSTI ID: 5914772
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 93:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHANNELING
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HERBS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ION CHANNELING
IONS
ISOTOPES
LANTHANUM COMPOUNDS
LEAVES
MEMBRANE TRANSPORT
MEMBRANES
NUCLEI
PHOSPHOHYDROLASES
PHYSIOLOGY
PLANTS
RADIOISOTOPES
RARE EARTH COMPOUNDS