Sequence similarities in the genes encoding polychlorinated biphenyl degradation by pseudomonas strain LB400 and alcaligenes eutrophus H850

Technical Report · Sun Jan 01 04:00:00 EST 1989

OSTI ID:5914335

Yates, J R; Mondello, F J

DNA-DNA hybridization was used to compare the Pseudomonas strain LB400 genes for polychlorinated biphenyl (PCB) degradation with those from seven other PCB-degrading strains. Significant hybridization was detected to the genome of Alcaligenes eutrophus H850, a strain similar to LB400 in PCB-degrading capability. These two organisms showed a strong conservation of restriction sites in the region of DNA encoding PCB metabolism. No other sequence similarities were detected in the two genomes. DNA from the other PCB-degrading strains showed no hybridization to the probe, which demonstrated the existence of at least two distinct classes of genes encoding PCB degradation.

• Research Organization: General Electric Co., Schenectady, NY (United States). Research and Development Center
• OSTI ID: 5914335
• Report Number(s): PB-93-229201/XAB
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
BACTERIA
BIOCHEMISTRY
BIODEGRADATION
BIOLOGY
BIOTECHNOLOGY
CHEMICAL REACTIONS
CHEMISTRY
CHLORINATED AROMATIC HYDROCARBONS
COMPARATIVE EVALUATIONS
DECOMPOSITION
DNA HYBRIDIZATION
EVALUATION
GENES
GENETIC ENGINEERING
GENETICS
HALOGENATED AROMATIC HYDROCARBONS
HYBRIDIZATION
METABOLISM
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDATION
POLYCHLORINATED BIPHENYLS
PSEUDOMONAS