Gene engineering in yeast for biodegradation: Immunological cross-reactivity among cytochrome p-450 system proteins of saccharomyces cerevisiae and candida tropicalis
Yeasts are eukaryotic microorganisms whose cytochrome P-450 monooxygenase systems may be amenable to genetic engineering for the hydroxylation and detoxication of polychlorinated aromatic hydrocarbons. The molecular genetic properties of strains of bakers yeast, Saccharomyces cerevisiae, and an n-alkane utilizing yeast, Candida tropicalis ATCC750 are examined. Standard methods were used to purify cytochrome P-450 and NADPH-cytochrome c (P-450) reductase proteins from cells cultured by semi-anaerobic glucose fermentation (S. cerevisiae, C. tropicalis) and by growth on tetradecane (C. tropicalis). Polyvalent antisera prepared in rabbits to some of these proteins were used in tests of immunological relatedness among the purified proteins using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and nitrocellulose filter immunoblots. The results provide evidence for gene relationships which should prove useful in gene isolation and subsequent engineering of P-450 enzyme systems in yeast.
- Research Organization:
- Cincinnati Univ. Medical Center, OH (United States)
- OSTI ID:
- 5914301
- Report Number(s):
- PB-93-229292/XAB; CNN: EPA-R-810605
- Resource Relation:
- Other Information: Pub. in Hazardous Waste and Hazardous Materials, Vol. 2, No. 2, 131-141(1985). See also PB--88-182597
- Country of Publication:
- United States
- Language:
- English
Similar Records
Oxygen requirements of yeasts. [Saccharomyces cerevisiae; Candida tropicalis]
Population genomics of the pathogenic yeast Candida tropicalis identifies hybrid isolates in environmental samples
Related Subjects
POLYCYCLIC AROMATIC HYDROCARBONS
BIODEGRADATION
SACCHAROMYCES CEREVISIAE
GENETIC ENGINEERING
ANAEROBIC CONDITIONS
CANDIDA
CYTOCHROMES
ELECTROPHORESIS
ENZYMES
FERMENTATION
GLUCOSE
HAZARDOUS MATERIALS
HYDROXYLATION
IMMUNE SERUMS
MICROORGANISMS
NITROCELLULOSE
PROTEINS
RABBITS
REACTIVITY
SODIUM
STRAINS
YEASTS
ALDEHYDES
ALKALI METALS
ANIMALS
AROMATICS
BIOCONVERSION
BIOTECHNOLOGY
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
ESTERS
EUMYCOTA
EXPLOSIVES
FUNGI
HEXOSES
HYDROCARBONS
MAMMALS
MATERIALS
METALS
MONOSACCHARIDES
NITRIC ACID ESTERS
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHAROMYCES
VERTEBRATES
550400* - Genetics
550700 - Microbiology