N-terminus conservation in the anchor polypeptide of a prokaryotic and eukaryotic alga. [Nostoc; Porphydium cruentum]
Energy flow between the extrinsic phycobilisomes and the photosystems within thylakoids, is probably mediated by a blue anchor polypeptide. Polypeptides in the 94 kD range, purified by LiDS-PAGE from phycobilisomes of Nostoc and Porphyrdium cruentum, crossreacted with anti-Nostoc-94 (although weakly with the latter). Though rich in ASP and GLU, the polypeptides were very hydrophobic, and low in MET, CYS, and HIS. Partial sequence of the N-terminus shows considerable homology 1 - 5 - 10 - 15 - 20 N: (S)-V-K-A-S-G-G-S-S-V-A-(R)-P-Q-L-Y-Q-(G)-L-(A)-V- P: V-()-K-A-S-G-G-S-P-V-V-K-P-Q-L-Y-(K)-()-A-(S)- between the species. There is a lack of homology when compared with ..cap alpha.. and ..beta.. polypeptides of allophycocyanin with rod linkers of phycobilisomes and other phycobiliproteins. Polypeptides of 94 and 92 kD from thylakoids of Nostoc, also immunoreactive with anti-94, were blocked at the N-terminus.
- Research Organization:
- Univ. of Maryland, College Park
- DOE Contract Number:
- AS05-76ER04310
- OSTI ID:
- 5905838
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALGAE
AMINO ACIDS
ASPARTIC ACID
AZOLES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHLOROPLASTS
CYSTEINE
DRUGS
ELECTROPHORESIS
GLUTAMIC ACID
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
IMMUNOASSAY
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
MEMBRANES
METHIONINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PHOTOSYNTHETIC MEMBRANES
PHYCOBILIPROTEINS
PHYCOBILISOMES
PHYCOCYANIN
PIGMENTS
PLANTS
POLYPEPTIDES
PROTEIN STRUCTURE
PROTEINS
THIOLS
THYLAKOID MEMBRANE PROTEINS