Laser-based optical-activity detector for high-performance liquid chromatography

Technical Report · Wed Jun 01 00:00:00 EDT 1983

OSTI ID:5904468

Kuo, J C

The identification of chromatographic peaks is simplified when detection is based on the rotation of polarized light by optically active molecules. The working system is based on the use of a laser and high quality optics. A detection limit approaching that of the conventional uv absorption detector can be achieved in a small volume flow cell. Three specific applications are discussed: in human urine, the determination of various sugars; in human plasma, the determination of the lipid profile; and the fractionation of shale oil, having different particle sizes and from different sources.

• Research Organization: Ames Lab., IA (USA)
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5904468
• Report Number(s): IS-T-1072; ON: DE83016118
• Resource Relation: Other Information: Thesis
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
04 OIL SHALES AND TAR SANDS
BLOOD SERUM
LIQUID COLUMN CHROMATOGRAPHY
CARBOHYDRATES
CHOLESTEROL
DETECTION
SHALE OIL
FRACTIONATION
URINE
LASERS
OPTICAL EQUIPMENT
PEAKS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHROMATOGRAPHY
ENERGY SOURCES
EQUIPMENT
FOSSIL FUELS
FUELS
HYDROXY COMPOUNDS
MATERIALS
MINERAL OILS
OILS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
SEPARATION PROCESSES
STEROIDS
STEROLS
WASTES
400105* - Separation Procedures
040500 - Oil Shales & Tar Sands- Properties & Composition