Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Translocation of an unusual cAMP receptor to the nucleus during development of Dictyostelium discoideum

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;
cAMP has been implicated in the control of the expression of developmental genes in Dictyostelium discoideum. To determine the potential role of cAMP receptors as regulators of gene expression, the authors have used immunocytochemical and immunoblotting techniques to reveal the subcellular localization of a cAMP binding protein CABP1. Most of the CABP1 antigen in early developing cells is localized near the cell periphery, with a small amount found in the nucleus. The level of CABP1 in the nucleus increases approx. = 30-fold during development. Moreover, immunofluorescence studies showed that CABP1 can also be detected on the cell surface. Binding of anti-CABP1 to intact cells followed by reaction with /sup 125/I-labeled secondary antibody revealed that the cell-surface CABP1 activity peaks during aggregation and culmination. In addition, several proteins related to CABP1 are found mainly in the nuclear fraction of developing cells. The possible role of these proteins in the regulation of development gene activity is discussed.
Research Organization:
McGill Univ., Montreal, Quebec
OSTI ID:
5903659
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:8; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Extracellular calmodulin regulates growth and cAMP-mediated chemotaxis in Dictyostelium discoideum
Journal Article · Fri Sep 07 00:00:00 EDT 2012 · Biochemical and Biophysical Research Communications · OSTI ID:22210227
Cloning and cDNA sequence of the regulator subunit of cAMP-dependent protein kinase from Dictyostelium discoideum
Journal Article · Wed Dec 31 23:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5607222
Studies of the cAMP mediated aggregation in Dictyostelium discoideum: receptor mediated activation of the adenylate cyclase
Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984 · OSTI ID:6068103

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL CONSTITUENTS
CELL DIFFERENTIATION
CELL NUCLEI
CHEMISTRY
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FLUORESCENCE
FUNGI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LUMINESCENCE
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
MYXOMYCETES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RADIOASSAY
RADIOISOTOPES
RECEPTORS